There were six positive tests for coronavirus across three Premier League clubs on Sunday and Monday, as the top flight prepares to resume in June.
The unnamed players or staff who have tested positive will now self-isolate for seven days.
A total of 748 players and staff from 19 clubs were tested. The remaining club did their tests on Tuesday so will be included in Saturday’s results.
Squads are starting non-contact training from Tuesday.
The Premier League has been suspended since 13 March because of the Covid-19 pandemic, with 92 fixtures remaining.